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Semisynthetic nanoreactor for reversible single-molecule covalent chemistry.

Abstract:

Protein engineering has been used to remodel pores for applications in biotechnology. For example, the heptameric α-hemolysin pore (αHL) has been engineered to form a nanoreactor to study covalent chemistry at the single-molecule level. Previous work has been confined largely to the chemistry of cysteine side chains or, in one instance, to an irreversible reaction of an unnatural amino acid side chain bearing a terminal alkyne. Here, we present four different αHL pores obtained by coupling ei...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acsnano.6b04663

Authors


Publisher:
American Chemical Society
Journal:
ACS Nano More from this journal
Volume:
10
Issue:
9
Pages:
8843-8850
Publication date:
2016-08-01
Acceptance date:
2016-08-18
DOI:
EISSN:
1936-086X
ISSN:
1936-0851
Pmid:
27537396
Language:
English
Keywords:
Pubs id:
pubs:640499
UUID:
uuid:3e8ab6d7-fe14-4303-afdc-1373e1e27fbf
Local pid:
pubs:640499
Source identifiers:
640499
Deposit date:
2017-10-19

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