The RNA polymerase of influenza a virus is stabilized by interaction with its viral RNA promoter.

Journal article

The RNA polymerase of the influenza virus is responsible for the transcription and replication of the segmented RNA viral genome during infection of host cells. Polymerase function is known to be strictly dependent on interaction with its RNA promoter, but no attempts to investigate whether the virion RNA (vRNA) promoter stabilizes the polymerase have been reported previously. Here we tested whether the vRNA promoter protects the polymerase against heat inactivation. We prepared partially pur...

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Authors: Brownlee, G; Sharps, J

• Publication status: Published
• Journal: Journal of virology
• Volume: 76
• Issue: 14
• Pages: 7103-7113
• Publication date: 2002-07-01
• DOI: 10.1128/jvi.76.14.7103-7113.2002
• EISSN: 1098-5514
• ISSN: 0022-538X
• Language: English
• Keywords: Nucleic Acid Conformation; RNA Replicase; Influenza A virus; Hot Temperature; Cell Line; Promoter Regions, Genetic; RNA, Viral; Base Sequence; Enzyme Stability; Base Pairing; Recombinant Proteins; Transcription, Genetic; Humans; Molecular Sequence Data