Elemental analysis of proteins by proton induced X-ray emission (microPIXE)

Journal article

The identification and quantification of metals bound to proteins is a crucial problem to be solved in structural biology. This chapter will describe the technique of proton induced X-ray emission with a microfocused proton beam (microPIXE) as a tool for analysing the elemental composition of liquid and crystalline protein samples. The proton beam induces characteristic X-ray emission from all elements in the protein, which can be interpreted in terms of the metal content of the protein molec...

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Authors: Garman, E; Zeldin, O

• Journal: NATO Science for Peace and Security Series A: Chemistry and Biology
• Pages: 79-89
• Publication date: 2013-01-01
• DOI: 10.1007/978-94-007-6232-9-8
• ISSN: 1874-6489
• Language: English
• Keywords: Structural genomics; Trace element analysis; Metalloproteins; microPIXE