Protease production during sporulation of germination mutants of Bacillus subtilis and the cloning of a functional gerE gene.

Journal article

Early in sporulation, cells of wild-type Bacillus subtilis produce three proteases (b, c and d) with monomeric Mr values of about 65 000, 53 000 and 43 500, and a further protease, e (Mr about 30 000) at the time of coat assembly. An additional protease, f (Mr about 15 000) appears transiently in sporangia at about the time of spore release. Three strains with defective spore coats were examined for alterations in sporulation proteases. A strain carrying the gerE36 mutation produces b, c and ...

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Authors: James, W; Mandelstam, J

• Publication status: Published
• Journal: Journal of general microbiology
• Volume: 131
• Issue: 9
• Pages: 2421-2430
• Publication date: 1985-09-01
• ISSN: 0022-1287
• Language: English
• Keywords: Mutation; Peptide Hydrolases; Genes, Bacterial; Electrophoresis, Polyacrylamide Gel; Cloning, Molecular; Bacillus subtilis; Spores, Bacterial; DNA Restriction Enzymes; DNA, Bacterial