The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells

Journal article

Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing efficient and successful cryopreservation methods for hES cell lines and banks. We investigated how the stimuli from cryopreservation result in apoptosis, which causes the low cell recovery rate after ...

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Authors: Xu, X; Cowley, S; Flaim, C; James, W; Seymour, L

• Journal: Biotechnology Progress
• Volume: 26
• Issue: 3
• Pages: 827-837
• Publication date: 2010-05-01
• DOI: 10.1002/btpr.368
• EISSN: 1520-6033
• ISSN: 8756-7938
• Language: English
• Keywords: Cryopreservation of hES cells; F-actin; Apoptosis inhibitors; Apoptosis pathways; Reactive oxygen species