Defining ELISpot cut-offs from unreplicated test and control wells.

Conference item

In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing transformation of the SFU counts, based on the Bland-Altman plot of the test and control wells. The second step is to derive a positivity threshold from the difference in between-plate distribution...

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Authors: Alexander, N; Fox, A; Lien, V; Dong, T; Lee, L

• Publication status: Published
• Host title: Journal of immunological methods
• Volume: 392
• Issue: 1-2
• Pages: 57-62
• Publication date: 2013-06-01
• Event location: Netherlands
• DOI: 10.1016/j.jim.2013.02.014
• EISSN: 1872-7905
• ISSN: 0022-1759
• Keywords: Adolescent; Cohort Studies; Young Adult; Vietnam; Analysis of Variance; Child, Preschool; Child; Aged; Humans; Peptides; Aged, 80 and over; Adult; Influenza, Human; Middle Aged; Enzyme-Linked Immunospot Assay