A novel cytosolic class I antigen-processing pathway for endoplasmic-reticulum-targeted proteins.

Journal article

Proteins bearing an endoplasmic reticulum (ER) leader are inserted into the ER followed by cleavage of the signal peptide. Major histocompatibility complex class I-restricted T-cell epitopes can be generated from these proteins by the proteasome after retrotranslocation into the cytosol. Here, we show that an HLA-A(*)0201-restricted epitope from prostate stem cell antigen contains the cleavage site of the ER signal peptidase. The resulting cleavage products fail to bind to HLA-A(*)0201 and ar...

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Authors: Schlosser, E; Otero, C; Wuensch, C; Kessler, B; Edelmann, M

• Publication status: Published
• Journal: EMBO reports
• Volume: 8
• Issue: 10
• Pages: 945-951
• Publication date: 2007-10-01
• DOI: 10.1038/sj.embor.7401065
• EISSN: 1469-3178
• ISSN: 1469-221X
• Language: English
• Keywords: GPI-Linked Proteins; Membrane Glycoproteins; Major Histocompatibility Complex; Antigens, Neoplasm; Endoplasmic Reticulum; T-Lymphocytes, Cytotoxic; Recombinant Fusion Proteins; Cell Line; Signal Transduction; Transfection; Membrane Proteins; Histocompatibility Antigens Class I; Antigen Presentation; Serine Endopeptidases; Neoplasm Proteins; Proteasome Endopeptidase Complex; Humans