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Journal article

Single-molecule protein phosphorylation and dephosphorylation by nanopore enzymology

Abstract:

Reversible protein phosphorylation plays a crucial and ubiquitous role in the control of almost all cellular processes. The interplay of protein kinases and phosphatases acting in opposition ensures tight dynamic control of protein phosphorylation states within the cell. Previously, engineered alpha-hemolysin pores bearing kinase substrate peptides have been developed as single-molecule stochastic sensors for protein kinases. Here, we have used these pores to observe, label-free, the phosphor...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acsnano.8b07697

Authors


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Role:
Author
ORCID:
0000-0002-6781-2367
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry
Role:
Author
ORCID:
0000-0003-2499-6116
Publisher:
American Chemical Society Publisher's website
Journal:
ACS Nano Journal website
Volume:
13
Issue:
1
Pages:
633-641
Publication date:
2018-12-27
Acceptance date:
2018-12-27
DOI:
EISSN:
1936-086X
ISSN:
1936-0851
Pmid:
30588793
Source identifiers:
963695
Language:
English
Keywords:
Pubs id:
pubs:963695
UUID:
uuid:3d987481-1741-494b-a9a1-99511ed79cca
Local pid:
pubs:963695
Deposit date:
2019-03-26

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