Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis.

Journal article

A cDNA coding for 3-ketoacyl-acyl-carrier protein (ACP) synthase III (KAS III) from spinach (Spinacia oleracea; So KAS III) was used to isolate two closely related KAS III clones (Ch KAS III-1 and Ch KAS III-2) from Cuphea hookeriana. Both Ch KAS IIIs are expressed constitutively in all tissues examined. An increase in the levels of 16:0 was observed in tobacco (Nicotiana tabacum, WT-SR) leaves overexpressing So KAS III when under the control of the cauliflower mosaic virus-35S promoter and i...

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Authors: Dehesh, K; Tai, H; Edwards, P; Byrne, J; Jaworski, J

• Publication status: Published
• Journal: Plant physiology
• Volume: 125
• Issue: 2
• Pages: 1103-1114
• Publication date: 2001-02-01
• DOI: 10.1104/pp.125.2.1103
• EISSN: 1532-2548
• ISSN: 0032-0889
• Language: English
• Keywords: DNA Primers; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Plant Proteins; Sequence Alignment; Tobacco; Gene Expression Regulation, Plant; Molecular Sequence Data; Gene Expression Regulation, Enzymologic; Kinetics; Plants, Genetically Modified; Reverse Transcriptase Polymerase Chain Reaction; Plant Oils; Plants; Sequence Homology, Amino Acid; Amino Acid Sequence; Lipids; Base Sequence; DNA, Complementary