A genome-scale metabolic model of Arabidopsis and some of its properties.

Journal article

We describe the construction and analysis of a genome-scale metabolic model of Arabidopsis (Arabidopsis thaliana) primarily derived from the annotations in the Aracyc database. We used techniques based on linear programming to demonstrate the following: (1) that the model is capable of producing biomass components (amino acids, nucleotides, lipid, starch, and cellulose) in the proportions observed experimentally in a heterotrophic suspension culture; (2) that approximately only 15% of the available reactions are needed for this purpose and that the size of this network is comparable to estimates of minimal network size for other organisms; (3) that reactions may be grouped according to the changes in flux resulting from a hypothetical stimulus (in this case demand for ATP) and that this allows the identification of potential metabolic modules; and (4) that total ATP demand for growth and maintenance can be inferred and that this is consistent with previous estimates in prokaryotes and yeast.

Authors: Poolman, MG; Miguet, L; Sweetlove, L; Fell, D

• Publication status: Published
• Journal: Plant physiology
• Volume: 151
• Issue: 3
• Pages: 1570-1581
• Publication date: 2009-11-01
• DOI: 10.1104/pp.109.141267
• EISSN: 1532-2548
• ISSN: 0032-0889
• Language: English
• Keywords: Genome, Plant; Cells, Cultured; Arabidopsis; Models, Biological; Adenosine Triphosphate; Biomass; Metabolome; Metabolic Networks and Pathways