Journal article
NEM modification prevents high-affinity ATP binding to the first nucleotide binding fold of the sulphonylurea receptor, SUR1.
- Abstract:
-
Pancreatic beta-cell ATP-sensitive potassium channels, composed of SUR1 and Kir6.2 subunits, serve as a sensor for intracellular nucleotides and regulate glucose-induced insulin secretion. To learn more about the interaction of SUR1 with nucleotides, we examined the effect of N-ethylmaleimide (NEM) modification. Photoaffinity labeling of SUR1 with 5 microM 8-azido-[alpha-32P]ATP or 8-azido-[gamma-32P]ATP was inhibited by NEM with Ki of 1.8 microM and 2.4 microM, and Hill coefficients of 0.94 ...
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- Publication status:
- Published
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- Publisher copy:
- 10.1016/s0014-5793(99)01170-9
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Bibliographic Details
- Journal:
- FEBS letters
- Volume:
- 458
- Issue:
- 3
- Pages:
- 292-294
- Publication date:
- 1999-09-01
- DOI:
- EISSN:
-
1873-3468
- ISSN:
-
0014-5793
- Source identifiers:
-
25855
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:25855
- UUID:
-
uuid:3d77a3c6-d18c-4305-bb3c-db29cf567838
- Local pid:
- pubs:25855
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 1999
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