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Blocking variant surface glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation.

Abstract:

BACKGROUND: The African trypanosome Trypanosoma brucei is covered with a dense layer of Variant Surface Glycoprotein (VSG), which protects it from lysis by host complement via the alternative pathway in the mammalian bloodstream. Blocking VSG synthesis by the induction of VSG RNAi triggers an unusually precise precytokinesis cell-cycle arrest. METHODOLOGY/PRINCIPAL FINDINGS: Here, we characterise the cells arrested after the induction of VSG RNAi. We were able to rescue the VSG221 RNAi induc...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1371/journal.pone.0007532

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Institution:
University of Oxford
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
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Institution:
University of Oxford
Role:
Author
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Institution:
University of Oxford
Role:
Author
Wellcome Trust More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PloS one Journal website
Volume:
4
Issue:
10
Article number:
e7532
Publication date:
2009-01-01
DOI:
EISSN:
1932-6203
ISSN:
1932-6203

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