Essential role of ubiquitin-specific protease 8 for receptor tyrosine kinase stability and endocytic trafficking in vivo.

Journal article

Posttranslational modification by ubiquitin controls multiple cellular functions and is counteracted by the activities of deubiquitinating enzymes. UBPy (USP8) is a growth-regulated ubiquitin isopeptidase that interacts with the HRS-STAM complex. Using Cre-loxP-mediated gene targeting in mice, we show that lack of UBPy results in embryonic lethality, whereas its conditional inactivation in adults causes fatal liver failure. The defect is accompanied by a strong reduction or absence of several...

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Authors: Niendorf, S; Oksche, A; Kisser, A; Löhler, J; Prinz, M

• Publication status: Published
• Journal: Molecular and cellular biology
• Volume: 27
• Issue: 13
• Pages: 5029-5039
• Publication date: 2007-07-01
• DOI: 10.1128/mcb.01566-06
• EISSN: 1098-5549
• ISSN: 0270-7306
• Language: English
• Keywords: Multiprotein Complexes; Cell Proliferation; Fibroblasts; Endosomal Sorting Complexes Required for Transport; Endopeptidases; Mutagenesis; Ubiquitin; Gene Targeting; Humans; Endosomes; Endocytosis; Mice; Gene Deletion; Death; Receptor, erbB-3; Animals; Ubiquitin Thiolesterase; Receptor Protein-Tyrosine Kinases; Liver; Enzyme Stability; Cell Line, Transformed; Embryo, Mammalian; Receptor, Epidermal Growth Factor; Proto-Oncogene Proteins c-met