High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples.

Journal article

The use of imino sugars for the potential treatment of lysosomal glycolipid storage diseases and hepatitis virus infections requires accurate, quantitative measurement of these compounds in biological samples. We demonstrate here the versatility of cation-exchange chromatography and pulsed amperometric detection of a range of compounds that differ in both isometric structure and N-alkyl chain length. Although column retention appears dependent upon residual charge on the imine function, succe...

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Authors: Mellor, H; Adam, A; Platt, F; Dwek, R; Butters, T

• Publication status: Published
• Journal: Analytical biochemistry
• Volume: 284
• Issue: 1
• Pages: 136-142
• Publication date: 2000-08-01
• DOI: 10.1006/abio.2000.4678
• EISSN: 1096-0309
• ISSN: 0003-2697
• Language: English
• Keywords: Chromatography, Ion Exchange; Liver; Carbohydrates; Electrochemistry; Models, Chemical; Time Factors; Brain; Lysosomal Storage Diseases; Cations; Mice; Mice, Inbred C57BL; Animals; 1-Deoxynojirimycin