Spacer-length dependence of programmed -1 or -2 ribosomal frameshifting on a U6A heptamer supports a role for messenger RNA (mRNA) tension in frameshifting.

Journal article

Programmed -1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slippery sequence, where the ribosome changes frame, and a stimulatory RNA structure, a stem-loop or RNA pseudoknot. Antisense oligonucleotides annealed appropriately 3' of a slipper...

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Authors: Lin, Z; Gilbert, R; Brierley, I

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 40
• Issue: 17
• Pages: 8674-8689
• Publication date: 2012-09-01
• DOI: 10.1093/nar/gks629
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Oligonucleotides, Antisense; COS Cells; Animals; Mass Spectrometry; Frameshifting, Ribosomal; Transfection; Protein Biosynthesis; Cercopithecus aethiops; RNA, Messenger