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Space and time-resolved gene expression experiments on cultured mammalian cells by a single-cell electroporation microarray.

Abstract:

Single-cell experiments represent the next frontier for biochemical and gene expression research. Although bulk-scale methods averaging populations of cells have been traditionally used to investigate cellular behavior, they mask individual cell features and can lead to misleading or insufficient biological results. We report on a single-cell electroporation microarray enabling the transfection of pre-selected individual cells at different sites within the same culture (space-resolved), at ar...

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Publisher copy:
10.1016/j.nbt.2008.03.002

Authors


Vassanelli, S More by this author
Bandiera, L More by this author
Cellere, G More by this author
Santoni, L More by this author
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Journal:
New biotechnology
Volume:
25
Issue:
1
Pages:
55-67
Publication date:
2008-06-05
DOI:
EISSN:
1876-4347
ISSN:
1871-6784
URN:
uuid:3c434388-f145-4a49-9c22-e40ae9382a78
Source identifiers:
237468
Local pid:
pubs:237468

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