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AAV-mediated gene transfer of human X-linked inhibitor of apoptosis protects against oxidative cell death in human RPE cells

Abstract:

Purpose. To determine whether human X-linked inhibitor of apoptosis (XIAP) enhances the survival of cultured human retinal pigment epithelial cells exposed to H 2O 2. Methods. ARPE-19 cells were exposed to H 2O 2 to induce oxidative cell death. Intracellular reactive oxygen species (ROS) were measured using 2′,7′-dichlorofluorescein diacetate. MTT assay was performed to quantify mitochondrial stress. Cell apoptosis was determined by TUNEL assay. Human XIAP was delivered with bicistronic expre...

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Publisher copy:
10.1167/iovs.10-6850

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience
Role:
Author
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Journal:
Investigative Ophthalmology and Visual Science
Volume:
52
Issue:
13
Pages:
9591-9597
Publication date:
2011-12-05
DOI:
EISSN:
1552-5783
ISSN:
0146-0404
URN:
uuid:3c33f4af-e969-4952-a502-d465fc90a3e3
Source identifiers:
344593
Local pid:
pubs:344593
Language:
English

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