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A T14C variant of Azotobacter vinelandii ferredoxin I undergoes facile [3Fe-4S]0 to [4Fe-4S]2+ conversion in vitro but not in vivo.

Abstract:

[4Fe-4S]2+/+ clusters that are ligated by Cys-X-X-Cys-X-X-Cys sequence motifs share the general feature of being hard to convert to [3Fe-4S]+/0 clusters, whereas those that contain a Cys-X-X-Asp-X-X-Cys motif undergo facile and reversible cluster interconversion. Little is known about the factors that control the in vivo assembly and conversion of these clusters. In this study we have designed and constructed a 3Fe to 4Fe cluster conversion variant of Azotobacter vinelandii ferredoxin I (FdI)...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.273.50.33692

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
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Journal:
Journal of biological chemistry
Volume:
273
Issue:
50
Pages:
33692-33701
Publication date:
1998-12-01
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
Source identifiers:
36775
Language:
English
Keywords:
Pubs id:
pubs:36775
UUID:
uuid:3c0b91ce-ee72-41de-a444-310e53e434e8
Local pid:
pubs:36775
Deposit date:
2013-11-17

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