Journal article
A procedure for setting up high-throughput nanolitre crystallization experiments. I. Protocol design and validation
- Abstract:
-
A protocol for setting up nanolitre sitting-drop vapour-diffusion experiments is reported. The procedure uses standard crystallization screening kits and 96-well crystallization plates. Reservoir solutions are transferred from 96-deep-well blocks to crystallization plates in a single step with a Robbins-Hydra pipettor. Nanolitre droplets of protein as well as reservoir solution are dispensed by a Cartesian pipetting instrument. Experiments have been carried out to characterize the performance...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- JOURNAL OF APPLIED CRYSTALLOGRAPHY
- Volume:
- 36
- Issue:
- 2
- Pages:
- 308-314
- Publication date:
- 2003-04-01
- DOI:
- ISSN:
-
0021-8898
Item Description
- Language:
- English
- Pubs id:
-
pubs:15474
- UUID:
-
uuid:3bee2fc1-8d9f-46da-a447-cab037e9dd0d
- Local pid:
- pubs:15474
- Source identifiers:
-
15474
- Deposit date:
- 2012-12-19
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- Copyright date:
- 2003
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