The roles of the glycosylphosphatidylinositol anchor on the production and immunogenicity of recombinant ookinete surface antigen Pbs21 of Plasmodium berghei when prepared in a baculovirus expression system.

Journal article

Malarial ookinetes express an immunodominant surface protein (P28) that is a priority candidate for the development of transmission-blocking vaccines. The full length P28 gene from Plasmodium berghei [Pbs21(1-213)] and a deletion construct [Pbs21(1-188)] encoding a protein that lacks the 25 C-terminal amino acids, including the glycosylphosphatidylinositol (GPI) anchor signal, were expressed in insect cells using baculovirus vectors. Pbs21(1-213) protein is strongly hydrophobic, found in the ...

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Authors: Martínez, A; Margos, G; Barker, G; Sinden, R

• Journal: Parasite immunology
• Volume: 22
• Issue: 10
• Pages: 493-500
• Publication date: 2000-10-01
• DOI: 10.1046/j.1365-3024.2000.00329.x
• EISSN: 1365-3024
• ISSN: 0141-9838
• Language: English
• Keywords: Animals; Antigens, Protozoan; Protozoan Proteins; Antigens, Surface; Chromatography, Affinity; Female; Cell Line; Glycosylphosphatidylinositols; Mice, Inbred BALB C; Plasmodium berghei; Genetic Vectors; Mice; Malaria; Recombinant Fusion Proteins; Gene Expression; Polymerase Chain Reaction; Baculoviridae