Journal article icon

Journal article

Single-cell sequencing of iPSC-dopamine neurons reconstructs disease progression and identifies HDAC4 as a regulator of Parkinson cell phenotypes

Abstract:

Induced pluripotent stem cell (iPSC)-derived dopamine neurons provide an opportunity to model Parkinson’s disease (PD), but neuronal cultures are confounded by asynchronous and heterogeneous appearance of disease phenotypes in vitro. Using high-resolution, single-cell transcriptomic analyses of iPSC-derived dopamine neurons carrying the GBA-N370S PD risk variant, we identified a progressive axis of gene expression variation leading to endoplasmic reticulum stress. Pseudotime analysis of genes...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1016/j.stem.2018.10.023

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
ORCID:
0000-0001-8190-4850
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDORMS
Subgroup:
KIR
Role:
Author
Expand authors...
More from this funder
Funding agency for:
Cowley, SA
More from this funder
Funding agency for:
Cowley, SA
Parkinson's UK More from this funder
National Institute for Health Research More from this funder
Expand funders...
Publisher:
Elsevier Publisher's website
Journal:
Cell Stem Cell Journal website
Volume:
24
Issue:
1
Pages:
93-106.e6
Publication date:
2018-11-29
Acceptance date:
2018-10-23
DOI:
EISSN:
1875-9777
ISSN:
1934-5909
Pubs id:
pubs:935895
URN:
uri:3abf1efd-cf05-41f8-9bd2-698a301ac521
UUID:
uuid:3abf1efd-cf05-41f8-9bd2-698a301ac521
Local pid:
pubs:935895

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP