Journal article
MEASUREMENTS OF CYTOPLASMIC PH IN INSULIN-RELEASING CELLS WITH INTRACELLULARLY TRAPPED PHENOL RED
- Abstract:
-
Cytoplasmic pH was measured in insulin-releasing clonal RINm5F cells using a new technique. The cells were permeabilized by electric discharges and loaded with phenol red. After resealing, changes in the absorbance of the intracellular indicator was monitored by dual wavelength spectrophotometry. The phenol red loading did not affect glucose metabolism, which was characterized by an oxidation to utilization ratio as low as 0.076. The basal cytoplasmic pH of the RINm5F cells was 7.25, and expo...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- BIOMEDICAL RESEARCH-TOKYO
- Volume:
- 7
- Issue:
- 3
- Pages:
- 139-144
- Publication date:
- 1986-06-01
- ISSN:
-
0388-6107
Item Description
- Language:
- English
- Pubs id:
-
pubs:19389
- UUID:
-
uuid:3a77f014-74e6-4738-a371-f8d366c9a9b6
- Local pid:
- pubs:19389
- Source identifiers:
-
19389
- Deposit date:
- 2012-12-19
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- Copyright date:
- 1986
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