Chondrogenic differentiation of murine embryonic stem cells: effects of culture conditions and dexamethasone.

Journal article

Pluripotent embryonic stem (ES) cells have the capability to differentiate to various cell types and may represent an alternative cell source for the treatment of cartilage defects. Here, we show that differentiation of ES cells toward the chondrogenic lineage can be enhanced by altering the culture conditions. Chondrogenesis was observed in intact embryoid body (EB) cultures, as detected by an increase in mRNA levels for aggrecan and Sox9 genes. Collagen IIB mRNA, the mature chondrocyte-spec...

Expand abstract

Authors: Tanaka, H; Murphy, C; Murphy, C; Kimura, M; Kawai, S

• Publication status: Published
• Journal: Journal of cellular biochemistry
• Volume: 93
• Issue: 3
• Pages: 454-462
• Publication date: 2004-10-01
• DOI: 10.1002/jcb.20171
• EISSN: 1097-4644
• ISSN: 0730-2312
• Language: English
• Keywords: Alginates; Dexamethasone; Aggrecans; Hexuronic Acids; Glucuronic Acid; Mice; Chondrocytes; Cell Differentiation; Animals; Proteoglycans; Stem Cells; Extracellular Matrix Proteins; Cells, Cultured; Cell Culture Techniques; Chondrogenesis; Lectins, C-Type; Transcription Factors; Collagen Type II; SOX9 Transcription Factor; High Mobility Group Proteins