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DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clones.

Abstract:

We have designed DOP-PCR primers specifically for the amplification of large insert clones for use in the construction of DNA microarrays. A bioinformatic approach was used to construct primers that were efficient in the general amplification of human DNA but were poor at amplifying E. coli DNA, a common contaminant of DNA preparations from large insert clones. We chose the three most selective primers for use in printing DNA microarrays. DNA combined from the amplification of large insert cl...

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Publisher copy:
10.1002/gcc.10155

Authors


Fiegler, H More by this author
Douglas, EJ More by this author
Burford, DC More by this author
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Journal:
Genes, chromosomes and cancer
Volume:
36
Issue:
4
Pages:
361-374
Publication date:
2003-04-05
DOI:
EISSN:
1098-2264
ISSN:
1045-2257
URN:
uuid:3a17b3a7-c0c4-44d1-956a-51ed38b53c09
Source identifiers:
72208
Local pid:
pubs:72208

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