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Primary microRNA transcripts are processed co-transcriptionally.

Abstract:

microRNAs (miRNAs) are generated from long primary (pri-) RNA polymerase II (Pol II)-derived transcripts by two RNase III processing reactions: Drosha cleavage of nuclear pri-miRNAs and Dicer cleavage of cytoplasmic pre-miRNAs. Here we show that Drosha cleavage occurs during transcription acting on both independently transcribed and intron-encoded miRNAs. We also show that both 5'-3' and 3'-5' exonucleases associate with the sites where co-transcriptional Drosha cleavage occurs, promoting int...

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Publisher copy:
10.1038/nsmb.1475

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
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Journal:
Nature structural and molecular biology
Volume:
15
Issue:
9
Pages:
902-909
Publication date:
2008-09-05
DOI:
EISSN:
1545-9985
ISSN:
1545-9993
URN:
uuid:3a0b8ea2-b960-46e5-b46a-3146a083984e
Source identifiers:
5223
Local pid:
pubs:5223

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