Journal article
A miniaturized screen for inhibitors of Jumonji histone demethylases.
- Abstract:
-
2-Oxoglutarate- and Fe(ii)-dependent oxygenases are a major class of N(epsilon)-methyl lysine demethylases that are involved in epigenetic regulation. Assays suitable for implementation in a high-throughput manner have been lacking for these enzymes. Here, we describe the design and implementation of a robust and miniaturized high-throughput kinetic assay for inhibitors of JMJD2E using a formaldehyde dehydrogenase-coupled reaction with real-time fluorescence detection. Reactant compatibility ...
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- Publication status:
- Published
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- Publisher copy:
- 10.1039/b912993f
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Authors
Bibliographic Details
- Journal:
- Molecular bioSystems
- Volume:
- 6
- Issue:
- 2
- Pages:
- 357-364
- Publication date:
- 2010-02-01
- DOI:
- EISSN:
-
1742-2051
- ISSN:
-
1742-206X
- Source identifiers:
-
48512
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:48512
- UUID:
-
uuid:39e3b467-afd9-4672-bdea-aff777e63c1a
- Local pid:
- pubs:48512
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2010
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