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Journal article

A miniaturized screen for inhibitors of Jumonji histone demethylases.

Abstract:

2-Oxoglutarate- and Fe(ii)-dependent oxygenases are a major class of N(epsilon)-methyl lysine demethylases that are involved in epigenetic regulation. Assays suitable for implementation in a high-throughput manner have been lacking for these enzymes. Here, we describe the design and implementation of a robust and miniaturized high-throughput kinetic assay for inhibitors of JMJD2E using a formaldehyde dehydrogenase-coupled reaction with real-time fluorescence detection. Reactant compatibility ...

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Publication status:
Published

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Publisher copy:
10.1039/b912993f

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Journal:
Molecular bioSystems
Volume:
6
Issue:
2
Pages:
357-364
Publication date:
2010-02-05
DOI:
EISSN:
1742-2051
ISSN:
1742-206X
URN:
uuid:39e3b467-afd9-4672-bdea-aff777e63c1a
Source identifiers:
48512
Local pid:
pubs:48512

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