Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells.

Journal article

The T cell receptor (TCR) expressed on most T cells is a protein complex consisting of TCRalphabeta heterodimers that bind antigen and cluster of differentiation (CD) 3epsilondelta, epsilongamma, and zetazeta dimers that initiate signaling. A long-standing controversy concerns whether there is one, or more than one, alphabeta heterodimer per complex. We used a form of single-molecule spectroscopy to investigate this question on live T cell hybridomas. The method relies on detecting coincident...

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Authors: James, JR; White, S; Clarke, R; Johansen, A; Dunne, P

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 104
• Issue: 45
• Pages: 17662-17667
• Publication date: 2007-11-01
• DOI: 10.1073/pnas.0700411104
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Antigens, CD86; Receptor-CD3 Complex, Antigen, T-Cell; Models, Molecular; Mice; Animals; Protein Subunits; Hybridomas; Antigens, CD28; Peptide Fragments; Receptors, Antigen, T-Cell; Dimerization; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocytes