Colicin E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on Escherichia coli ribosomes.

Journal article

The cytotoxin colicin E3 targets the 30S subunit of bacterial ribosomes and specifically cleaves 16S rRNA at the decoding centre, thereby inhibiting translation. Although the cleavage site is well known, it is not clear which step of translation is inhibited. We studied the effects of colicin E3 cleavage on ribosome functions by analysing individual steps of protein synthesis. We find that the cleavage affects predominantly the elongation step. The inhibitory effect of colicin E3 cleavage ori...

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Authors: Lancaster, L; Savelsbergh, A; Kleanthous, K; Wintermeyer, W; Rodnina, M

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 69
• Issue: 2
• Pages: 390-401
• Publication date: 2008-07-01
• DOI: 10.1111/j.1365-2958.2008.06283.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Escherichia coli; Ribosomes; RNA, Bacterial; Colicins; RNA, Transfer; RNA, Ribosomal, 16S; Models, Biological; Protein Biosynthesis; Electrophoresis, Polyacrylamide Gel