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Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.

Abstract:

Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated depletion of the marker's fluorescent state by stimulated emission, but this approach requires picosecond laser pulses of GW/cm2 intensity. Here, we demonstrate the surpassing of the diffraction barrier in fluorescence microscopy with illumination intensities that are eight orders of magn...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.0506010102

Authors


Hofmann, M More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
102
Issue:
49
Pages:
17565-17569
Publication date:
2005-12-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:39a26593-0bb0-4e3c-b306-664e7d02c081
Source identifiers:
222163
Local pid:
pubs:222163

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