- Abstract:
-
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show th...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1021/nl204535h
-
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- Journal:
- Nano letters
- Volume:
- 12
- Issue:
- 5
- Pages:
- 2260-2265
- Publication date:
- 2012-05-05
- DOI:
- EISSN:
-
1530-6992
- ISSN:
-
1530-6984
- URN:
-
uuid:38e97366-4e0c-436a-9a68-ea3f16f19e69
- Source identifiers:
-
320343
- Local pid:
- pubs:320343
- Language:
- English
- Keywords:
- Copyright date:
- 2012
Journal article
Lipid-based passivation in nanofluidics.
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