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Wavelength considerations in confocal microscopy of botanical specimens

Abstract:

We have used a multiple-laser confocal microscope with lines 325, 442, 488, 514 and 633 nm to investigate optical sectioning of botanical specimens over a wide range of wavelengths. The 442-nm line allowed efficient excitation of Chromomycin A3, with minimal background autofluorescence, to visualize GC-rich heterochromatin as an aid to chromosome identification. Sequential excitation with 442- and 488-nm light enabled ratio imaging of cytosolic pH using BCECF. The red HeNe laser penetrated de...

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Publication status:
Published
Peer review status:
Peer reviewed

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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Role:
Author
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Funding agency for:
Fricker, M
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Funding agency for:
Fricker, M
Publisher:
Wiley Publisher's website
Journal:
Journal of Microscopy Journal website
Volume:
166
Issue:
1
Pages:
29-42
Publication date:
1992-04-05
DOI:
ISSN:
0022-2720
URN:
uuid:38d0a39d-caf8-4e6b-8a84-faa96ee58ddd
Source identifiers:
43780
Local pid:
pubs:43780

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