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Rad25p, a DNA helicase subunit of yeast transcription factor TFIIH, is required for promoter escape in vivo.

Abstract:

The general transcription factor TFIIH is required for initial DNA unwinding and promoter escape by RNA polymerase II in vitro. We examined whether Rad25p, a DNA helicase subunit of TFIIH, mediates promoter opening and promoter escape in the yeast Saccharomyces cerevisiae. DNA unwinding was probed with an in vivo permanganate reactivity assay, in a temperature-sensitive mutant of RAD25. The consequences of Rad25p inactivation were promoter-specific. Whereas in the TDH2 promoter permanganate r...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Role:
Author
Journal:
Gene
Volume:
245
Issue:
1
Pages:
109-117
Publication date:
2000-03-05
DOI:
EISSN:
1879-0038
ISSN:
0378-1119
URN:
uuid:38bacd1f-b49d-48ea-843a-455f04d47071
Source identifiers:
31640
Local pid:
pubs:31640

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