- Abstract:
- In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.
- Publisher copy:
- 10.1007/978-1-62703-764-8_15
-
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- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Volume:
- 1116
- Pages:
- 209-234
- Publication date:
- 2014
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- URN:
-
uuid:38ba9e72-654d-46cc-9636-a5bc1acf82de
- Source identifiers:
-
445677
- Local pid:
- pubs:445677
- Language:
- English
- Keywords:
- Copyright date:
- 2014
Journal article
Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.
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