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Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

Abstract:
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
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Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
1116
Pages:
209-234
Publication date:
2014-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
445677
Language:
English
Keywords:
Pubs id:
pubs:445677
UUID:
uuid:38ba9e72-654d-46cc-9636-a5bc1acf82de
Local pid:
pubs:445677
Deposit date:
2014-02-07

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