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Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

Abstract:
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.

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Authors


Flanagan, J More by this author
Diprose, JM More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
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Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
1116
Pages:
209-234
Publication date:
2014
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:38ba9e72-654d-46cc-9636-a5bc1acf82de
Source identifiers:
445677
Local pid:
pubs:445677

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