Non-homologous end-joining partners in a helical dance: structural studies of XLF-XRCC4 interactions.

Journal article

XRCC4 (X-ray cross-complementation group 4) and XLF (XRCC4-like factor) are two essential interacting proteins in the human NHEJ (non-homologous end-joining) pathway that repairs DNA DSBs (double-strand breaks). The individual crystal structures show that the dimeric proteins are homologues with protomers containing head domains and helical coiled-coil tails related by approximate two-fold symmetry. Biochemical, mutagenesis, biophysical and structural studies have identified the regions of in...

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Authors: Wu, Q; Ochi, T; Matak-Vinkovic, D; Robinson, C; Chirgadze, D

• Publication status: Published
• Journal: Biochemical Society transactions
• Volume: 39
• Issue: 5
• Pages: 1387-1392
• Publication date: 2011-10-01
• DOI: 10.1042/bst0391387
• EISSN: 1470-8752
• ISSN: 0300-5127
• Language: English
• Keywords: Protein Binding; DNA Repair Enzymes; Protein Structure, Quaternary; Dimerization; DNA Repair; Humans; DNA-Binding Proteins; DNA End-Joining Repair; Protein Structure, Secondary; DNA Breaks, Double-Stranded; Models, Molecular; DNA Ligases; Crystallization; DNA