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Aberration-free optical refocusing in high numerical aperture microscopy.

Abstract:
We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 mum for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen.
Publication status:
Published

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Publisher copy:
10.1364/ol.32.002007

Authors


Botcherby, EJ More by this author
Juskaitis, R More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Journal:
Optics letters
Volume:
32
Issue:
14
Pages:
2007-2009
Publication date:
2007-07-05
DOI:
EISSN:
1539-4794
ISSN:
0146-9592
URN:
uuid:383f7173-d481-406e-9bf0-dd7117048798
Source identifiers:
62533
Local pid:
pubs:62533

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