Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides.

Journal article

The Xenopus laevis oocyte offers one of the most convenient expression systems for assaying the actions of candidate ligands on cloned ionotropic neurotransmitter receptors (also known as ligand-gated ion channels [LGICs]). Their large size makes injection of complementary ribonucleic acid or complementary deoxyribonucleic acid and electrophysiological recording very easy. Furthermore, Xenopus oocytes translate messages very efficiently, resulting in the detection of large-amplitude ligand-in...

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Authors: Buckingham, S; Pym, L; Sattelle, D

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 322
• Pages: 331-345
• Publication date: 2006-01-01
• DOI: 10.1007/978-1-59745-000-3_23
• EISSN: 1940-6029
• ISSN: 1064-3745
• Language: English
• Keywords: Receptors, Neurotransmitter; Insecticides; Xenopus laevis; Female; Animals; Ligands; Patch-Clamp Techniques; Oocytes; Ion Channel Gating