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Site of attachment of retinal in bacteriorhodopsin.

Abstract:

The identity of the lysine residue in bacteriorhodopsin to which the chromophore, retinal, is attached as a Schiff's base has been reinvestigated. Retinal is now shown to be linked to lysine-216 and not lysine-41, as had been concluded previously. The retinal in purple membrane was replaced by [15-3H]retinal, the Schiff's base linkage was reduced with NaBH4 while the sample was illuminated, and the resulting [retinyl-3H]bacterio-opsin was cleaved with CNBr. The radioactivity was present exclu...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.78.4.2225

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Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
78
Issue:
4
Pages:
2225-2229
Publication date:
1981-04-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:379f0f9e-0e05-4063-b5f7-29ad22424e2d
Source identifiers:
52414
Local pid:
pubs:52414

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