Analyses of MT1-MMP activity in cells.

Journal article

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a type I transmembrane protein which exhibits biological activities on the cell surface. One of the characteristic functions of this enzyme is activation of proMMP-2 on the cell surface (1). This process can be monitored using gelatin zymography to detect the pro- and active forms of MMP-2. Cellular MT1-MMP activity can also be directly detected by in situ degradation of layers of either gelatin or collagen. By combining inhibitors, wester...

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Authors: Evans, R; Itoh, Y

• Journal: Methods in molecular medicine
• Volume: 135
• Pages: 239-249
• Publication date: 2007-01-01
• EISSN: 1940-6037
• ISSN: 1543-1894
• Language: English
• Keywords: Cell Line; Enzyme Precursors; Metalloendopeptidases; Enzyme Activation; Immunohistochemistry; Collagen Type I; Blotting, Western; Tissue Inhibitor of Metalloproteinase-2; Gelatin; Gelatinases; Humans; Protease Inhibitors; Dipeptides; Matrix Metalloproteinase 14; Arthritis, Rheumatoid; Fibroblasts; Synovial Membrane; Fluorescent Antibody Technique; Tissue Inhibitor of Metalloproteinase-1