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Multiplexed proteomics mapping of yeast RNA polymerase II and III allows near-complete sequence coverage and reveals several novel phosphorylation sites.

Abstract:

The multisubunit RNA polymerases (Pols) II and III synthesize mainly eukaryotic mRNAs and tRNAs, respectively. Pol II and Pol III are protein complexes consisting of 12 and 17 subunits. Here we analyzed both yeast Pol II and Pol III by multiplexed mass spectrometric analysis using various proteases and both collision induced and electron transfer dissociation. The cumulative data obtained from using the various proteases (trypsin, chymotrypsin, Glu-C and Lys-C) and the two peptide fragmentati...

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Publication status:
Published

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Publisher copy:
10.1021/ac7024283

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
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Journal:
Analytical chemistry
Volume:
80
Issue:
10
Pages:
3584-3592
Publication date:
2008-05-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:36c39c94-c561-4b46-a9ff-2c6573533671
Source identifiers:
384604
Local pid:
pubs:384604

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