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Journal article

Following evolutionary paths to protein-protein interactions with high affinity and selectivity.

Abstract:

How do intricate multi-residue features such as protein-protein interfaces evolve? To address this question, we evolved a new colicin-immunity binding interaction. We started with Im9, which inhibits its cognate DNase ColE9 at 10(-14) M affinity, and evolved it toward ColE7, which it inhibits promiscuously (Kd > 10(-8) M). Iterative rounds of random mutagenesis and selection toward higher affinity for ColE7, and selectivity (against ColE9 inhibition), led to an approximately 10(5)-fold inc...

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Publication status:
Published

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Publisher copy:
10.1038/nsmb.1670

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Journal:
Nature structural and molecular biology
Volume:
16
Issue:
10
Pages:
1049-1055
Publication date:
2009-10-01
DOI:
EISSN:
1545-9985
ISSN:
1545-9993
Source identifiers:
310217
Language:
English
Keywords:
Pubs id:
pubs:310217
UUID:
uuid:366445a9-d252-41e8-bb09-79998a288855
Local pid:
pubs:310217
Deposit date:
2012-12-19

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