Journal article icon

Journal article

Sample preparation and mass-spectrometric characterization of crystal-derived protein samples.

Abstract:

Mass spectrometry is often used to ascertain the accurate mass of purified protein samples prior to crystallization screening. However, in many cases data regarding the form of the protein crystallizing can also be useful, as this may differ from the original sample. Development of a simple method for the preparation and mass spectrometry of crystal-derived protein samples is described. The method is exemplified by the determination of the phosphorylation state of protein in a crystal derived...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1107/s0907444905007262

Authors


Nettleship, JE More by this author
Walter, TS More by this author
Stammers, DK More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
61
Issue:
Pt 5
Pages:
643-645
Publication date:
2005-05-05
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
URN:
uuid:362a9a56-9015-4fcc-9b6d-7786bd5d7e44
Source identifiers:
33077
Local pid:
pubs:33077

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP