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Journal article

Sample preparation and mass-spectrometric characterization of crystal-derived protein samples.

Abstract:

Mass spectrometry is often used to ascertain the accurate mass of purified protein samples prior to crystallization screening. However, in many cases data regarding the form of the protein crystallizing can also be useful, as this may differ from the original sample. Development of a simple method for the preparation and mass spectrometry of crystal-derived protein samples is described. The method is exemplified by the determination of the phosphorylation state of protein in a crystal derived...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444905007262

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
61
Issue:
Pt 5
Pages:
643-645
Publication date:
2005-05-01
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Source identifiers:
33077
Language:
English
Keywords:
Pubs id:
pubs:33077
UUID:
uuid:362a9a56-9015-4fcc-9b6d-7786bd5d7e44
Local pid:
pubs:33077
Deposit date:
2012-12-19

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