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Restriction site-specific methylation studies of imprinted genes with quantitative real-time PCR.

Abstract:

BACKGROUND: Epigenetic studies, such as the measurement of DNA methylation, are important in the investigation of syndromes influenced by imprinted genes. Quick and accurate quantification of methylation at such genes can be of appreciable diagnostic aid. METHODS: We first digested genomic DNA with methylation-sensitive restriction enzymes and used DNA without digestion as a control and nonmethylated lambda DNA as an internal control for digestion efficiency. We then performed quantitative r...

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Publication status:
Published

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Authors


Hannula-Jouppi, K More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, WTC Human Genetics
Lipsanen-Nyman, M More by this author
Journal:
Clinical chemistry
Volume:
54
Issue:
3
Pages:
491-499
Publication date:
2008-03-05
DOI:
EISSN:
1530-8561
ISSN:
0009-9147
URN:
uuid:35cf9022-9a39-4e15-a805-0196ff37d231
Source identifiers:
6441
Local pid:
pubs:6441

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