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Journal article

Restriction site-specific methylation studies of imprinted genes with quantitative real-time PCR.

Abstract:: BACKGROUND: Epigenetic studies, such as the measurement of DNA methylation, are important in the investigation of syndromes influenced by imprinted genes. Quick and accurate quantification of methylation at such genes can be of appreciable diagnostic aid. METHODS: We first digested genomic DNA with methylation-sensitive restriction enzymes and used DNA without digestion as a control and nonmethylated lambda DNA as an internal control for digestion efficiency. We then performed quantitative r...
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Publication status:: Published

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APA Style

Bruce, S., Hannula-Jouppi, K., Lindgren, C., Lipsanen-Nyman, M., & Kere, J. (2008). Restriction site-specific methylation studies of imprinted genes with quantitative real-time PCR. Clinical Chemistry, 54(3), 491–499.

MLA Style

Bruce, S., et al. “Restriction Site-Specific Methylation Studies of Imprinted Genes with Quantitative Real-Time PCR.” Clinical Chemistry, vol. 54, no. 3, 2008, pp. 491–99.

Chicago Style

Bruce, S, K Hannula-Jouppi, C Lindgren, M Lipsanen-Nyman, and J Kere. 2008. “Restriction Site-Specific Methylation Studies of Imprinted Genes with Quantitative Real-Time PCR.” Clinical Chemistry 54 (3): 491–99.
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Publisher copy:: 10.1373/clinchem.2007.098491

Authors

+ Bruce, S More by this author

Role:

Author

+ Hannula-Jouppi, K More by this author

Role:

Author

+ Lindgren, C More by this author

Institution:

University of Oxford

Division:

MSD

Department:

NDM

Sub department:

Human Genetics Wt Centre

Role:

Author

+ Lipsanen-Nyman, M More by this author

Role:

Author

+ Kere, J More by this author

Role:

Author

Bibliographic Details

Journal:: Clinical chemistry
Volume:: 54
Issue:: 3
Pages:: 491-499
Publication date:: 2008-03-01
DOI:: 10.1373/clinchem.2007.098491
EISSN:: 1530-8561
ISSN:: 0009-9147

Item Description

Language:: English
Keywords:: DNA Methylation

Female

Genetic Diseases, Inborn

Genomic Imprinting

Polymerase Chain Reaction

Uniparental Disomy

Chromosomes, Human, Pair 7

Chromosomes, Human, Pair 11

Male

Humans
Pubs id:: pubs:6441
UUID:: uuid:35cf9022-9a39-4e15-a805-0196ff37d231
Local pid:: pubs:6441
Source identifiers:: 6441
Deposit date:: 2012-12-19

Terms of use

Copyright date:: 2008

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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