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Thesis

The biochemistry of antigen presentation

Abstract:

This thesis describes studies on the binding of peptides to the murine major histocompatibility complex (MHC) class I molecule H-2Db (Db). The expression of the recombinant soluble Db molecule in Chinese hamster ovary cells and its subsequent purification by nickel affinity chromatography, gel filtration, and preparative native isoelectric focusing are reported. The product is the correct molecule, homogeneous, a dimer of dimers, and free of endogenous pep...

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Institution:
University of Oxford
Department:
Institute of Molecular Medicine
Publication date:
1996
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
Barcode:
603824887
URN:
uuid:34d1afd2-fafc-4732-8e43-e00dcd8460d1
Local pid:
td:603824887

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