- Abstract:
-
This thesis describes studies on the binding of peptides to the murine major histocompatibility complex (MHC) class I molecule H-2Db (Db). The expression of the recombinant soluble Db molecule in Chinese hamster ovary cells and its subsequent purification by nickel affinity chromatography, gel filtration, and preparative native isoelectric focusing are reported. The product is the correct molecule, homogeneous, a dimer of dimers, and free of endogenous pep...
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- Publication date:
- 1996
- Type of award:
- DPhil
- Level of award:
- Doctoral
- Awarding institution:
- University of Oxford
- URN:
-
uuid:34d1afd2-fafc-4732-8e43-e00dcd8460d1
- Local pid:
- td:603824887
- Language:
- English
- Subjects:
- Copyright holder:
- Springer, Sebastian Hartmut
- Copyright date:
- 1996
- Notes:
- The digital copy of this thesis has been made available thanks to the generosity of Dr Leonard Polonsky
Thesis
The biochemistry of antigen presentation
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