Journal article
Reassessment of antibody-based detection of the murine T cell GLP-1 receptor
- Abstract:
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Glucagon-like peptide 1 receptor (GLP-1R) agonists exhibit anti-inflammatory actions, yet the importance of direct immune cell GLP-1R signaling remains uncertain. Although T cells respond to GLP-1, low receptor abundance and suboptimal antisera complicate efforts to characterize immune cell GLP-1R signaling. Here, we evaluate three frequently utilized GLP-1R antibodies, revealing that one of several antibodies, AGR-021, lack ideal specificity for detecting the GLP-1R in mice. Immunostaining with AGR-021 using tissues from two independent GLP-1R knockout mouse lines reveals persistent immunoreactive signals in GLP-1R-null pancreatic islets. Similarly, flow cytometry using AGR-021 reveals no reduction in AGR-021 immunoreactivity in GLP-1R-null splenic T cells. Moreover, western blotting detects AGR-021-immunoreactive proteins from a GLP-1R-negative cell line and fails to detect immunoreactive GLP-1R of the correct size upon overexpression of the receptor. Our findings reveal caveats governing use of multiple widely used GLP-1R antibodies, reemphasizing the importance of rigorous antibody validation for inferring accurate GLP-1R expression.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1016/j.cmet.2025.06.012
Authors
- Funder identifier:
- https://ror.org/050rgn017
- Grant:
- 24/0006744
- 22/0006389
- 23/0006627
- Funder identifier:
- https://ror.org/00vqxjy61
- Grant:
- 1-PNF-2025-1623-S-B
- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- APP23529
- Funder identifier:
- https://ror.org/001aqnf71
- Grant:
- EP/X026833/1
- Publisher:
- Cell Press
- Journal:
- Cell Metabolism More from this journal
- Volume:
- 37
- Issue:
- 9
- Pages:
- 1783-1788
- Publication date:
- 2025-09-02
- Acceptance date:
- 2025-06-23
- DOI:
- EISSN:
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1932-7420
- ISSN:
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1550-4131
- Language:
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English
- Pubs id:
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2282602
- Local pid:
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pubs:2282602
- Deposit date:
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2025-08-20
Terms of use
- Copyright holder:
- Elsevier Inc.
- Copyright date:
- 2025
- Rights statement:
- Copyright: © 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
- Notes:
- The author accepted manuscript (AAM) of this paper has been made available under the University of Oxford's Open Access Publications Policy, and a CC BY public copyright licence has been applied.
- Licence:
- CC Attribution (CC BY)
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