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Engineering the CYP101 system for in vivo oxidation of unnatural substrates.

Abstract:

The protein engineering of CYP enzymes for structure-activity studies and the oxidation of unnatural substrates for biotechnological applications will be greatly facilitated by the availability of functional, whole-cell systems for substrate oxidation. We report the construction of a tricistronic plasmid that expresses the CYP101 monooxygenase from Pseudomonas putida, and its physiological electron transfer co-factor proteins putidaredoxin reductase and putidaredoxin in Escherichia coli, givi...

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Publication status:
Published

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Publisher copy:
10.1093/protein/14.10.797

Authors


Harford-Cross, CF More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Journal:
Protein engineering
Volume:
14
Issue:
10
Pages:
797-802
Publication date:
2001-10-05
DOI:
EISSN:
1460-213X
ISSN:
0269-2139
URN:
uuid:346f46c1-924b-420e-9e51-12008efb5438
Source identifiers:
32008
Local pid:
pubs:32008

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