Journal article
Heterologously expressed polypeptide from the yeast meiotic gene HOP1 binds preferentially to yeast DNA.
- Abstract:
-
HOP1 protein, present in sporulating cells of Saccharomyces cerevisiae and believed to be a component of the synaptonemal complex, has been expressed in Escherichia coli fused to a biotinylated tag protein. Once solubilized from bacterial inclusion bodies, the HOP1 fusion protein was purified by using a combination of avidin-affinity chromatography and gel filtration FPLC and refolded. Sequence comparisons indicate that the HOP1 gene product contains a zinc finger motif, which may confer DNA ...
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- Publication status:
- Published
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- Publisher copy:
- 10.1006/prep.1999.1052
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Bibliographic Details
- Journal:
- Protein expression and purification
- Volume:
- 16
- Issue:
- 2
- Pages:
- 251-260
- Publication date:
- 1999-07-01
- DOI:
- EISSN:
-
1096-0279
- ISSN:
-
1046-5928
- Source identifiers:
-
53208
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:53208
- UUID:
-
uuid:343f1c68-9f60-4619-a266-b3d05de0b09d
- Local pid:
- pubs:53208
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 1999
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