Matrix metalloproteinases and TIMPS in cultured C57BL/6J-cpk kidney tubules.

Journal article

Restructuring of basement membranes is a hallmark of the pathology of renal cystic disorders. Here, we present findings consistent with the view that basement membrane degradation by matrix metallo-proteinases (MMPs) may contribute to abnormal basement membrane structure in polycystic kidney disease. Cells from cystic kidney tubules embedded in collagen gels appeared to migrate through the gel. This migration through collagen indicated that these cells could degrade the matrix. To examine thi...

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Authors: Rankin, C; Suzuki, K; Itoh, Y; Ziemer, D; Grantham, J

• Journal: Kidney international
• Volume: 50
• Issue: 3
• Pages: 835-844
• Publication date: 1996-09-01
• DOI: 10.1038/ki.1996.383
• EISSN: 1523-1755
• ISSN: 0085-2538
• Language: English
• Keywords: Sulfhydryl Reagents; Cells, Cultured; Cell Movement; Basement Membrane; Blotting, Northern; Mice; Glycoproteins; Matrix Metalloproteinase 3; Animals; Protease Inhibitors; Kidney Diseases, Cystic; Tissue Inhibitor of Metalloproteinase-2; Male; Phenylmercuric Acetate; Mice, Mutant Strains; Matrix Metalloproteinase 2; Metalloendopeptidases; RNA, Messenger; Culture Media, Conditioned; Matrix Metalloproteinase 9; Tissue Inhibitor of Metalloproteinases; Collagenases; Kidney Tubules; Gelatinases; Extracellular Matrix Proteins; Female; Mice, Inbred C57BL; Proteins