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Single-molecule detection by two-photon excitation of fluorescence

Abstract:

Using a mode-locked titanium: sapphire laser at 700 nm for two-photon excitation we studied fluorescence bursts from individual coumarin 120 molecules in water and triacetin. Fluorescence lifetimes and multichannel scaler traces have been measured simultaneously. Due to the fact that scattered excitation light as well as Raman scattered photons can be suppressed by a short-pass filter a very low background level was achieved. To identify the fluorophore by its characteristic fluorescence life...

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Publication status:
Published

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Publisher copy:
10.1117/12.273568

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
Journal:
ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY III
Volume:
2980
Pages:
552-558
Publication date:
1997-01-01
DOI:
ISSN:
0277-786X
URN:
uuid:33f0dddc-e41f-4ae5-92e3-81a69269f4a5
Source identifiers:
222218
Local pid:
pubs:222218

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