Journal article icon

Journal article

Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog

Abstract:

Background. A novel fluorescent cAMP analog (8-[Pharos-575]- adenosine-3′, 5′-cyclic monophosphate) was characterized with respect to its spectral properties, its ability to bind to and activate three main isoenzymes of the cAMP-dependent protein kinase (PKA-Iα, PKA-IIα, PKA-IIβ) in vitro, its stability towards phosphodiesterase and its ability to permeate into cultured eukaryotic cells using resonance energy transfer based indicators, and conventional fluorescence imaging. Results. The Pharo...

Expand abstract

Actions


Access Document


Publisher copy:
10.1186/1471-2091-9-18

Authors


Journal:
BMC Biochemistry
Volume:
9
Issue:
1
Publication date:
2008-01-01
DOI:
EISSN:
1471-2091
Source identifiers:
238916
Language:
English
Pubs id:
pubs:238916
UUID:
uuid:33c35f31-7689-4e66-b675-1a4361105d75
Local pid:
pubs:238916
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP