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Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression

Abstract:

Toll like receptors (TLRs) are important pattern recognition receptors that can detect pathogen and danger associated molecular patterns to initiate an innate immune response. TLR1 and 2 heterodimerize at the plasma membrane upon binding to triacylated lipopeptides from bacterial cell walls, or to the synthetic ligand Pam3CSK4. TLR1/2 dimers interact with adaptor molecules TIRAP and MyD88 to initiate a signalling cascade that leads to activation of key transcription factors, including NF-kB. ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.pone.0202408

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM - Investigative Medicine Division
ORCID:
0000-0002-8396-1354
Schofield, L More by this author
Pfleger, KDG More by this author
Eriksson, EM More by this author
Victorian State Government More from this funder
Australian National Health and Medical Research Council More from this funder
Australian Research Council More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PLoS One Journal website
Volume:
13
Issue:
8
Pages:
e0202408
Publication date:
2018-08-23
Acceptance date:
2018-08-02
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
Pubs id:
pubs:911178
URN:
uri:339d39cb-df61-4285-9f95-12a19d8b7543
UUID:
uuid:339d39cb-df61-4285-9f95-12a19d8b7543
Local pid:
pubs:911178
Language:
English
Keywords:

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